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polyclonal anti porcine igm antibody  (Bio-Rad)


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    Bio-Rad polyclonal anti porcine igm antibody
    Polyclonal Anti Porcine Igm Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+porcine+igm+antibody/Goat+anti+Pig+IgM/pm41774768-161-39-44
    Average 94 stars, based on 36 article reviews
    polyclonal anti porcine igm antibody - by Bioz Stars, 2026-10
    94/100 stars

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    other:

    Article Title: Duration of immunity following infection with moderately virulent ASFV.
    Article Snippet: All sera were diluted with washing solution (1 × PBS supplemented with 0.05% Tween-20 (Roth)) at a ratio of 1:30 immediately prior to performing the experiment.

    Incubation:

    Article Title: Duration of immunity following infection with moderately virulent ASFV
    Article Snippet: .. After incubation and subsequent washing, the respective, HRP-conjugated secondary antibody was added to each well: for (I) IgG detection, a polyclonal anti-porcine IgG antibody (#A100-205P, Biomol, Hamburg, Germany) was added at a dilution of 1:10,000, whereas for (II) IgM detection, a polyclonal anti-porcine IgM antibody (#AAI48, Bio-Rad, Feldkirchen, Germany) was added at a dilution of 1:5,000. .. The signal was acquired with an ELISA reader (TECAN, Switzerland).

    Article Title: Duration of immunity following infection with moderately virulent ASFV
    Article Snippet: .. After incubation and subsequent washing, the respective, HRP-conjugated secondary antibody was added to each well: for (I) IgG detection, a polyclonal anti-porcine IgG antibody (#A100-205P, Biomol) was added at a dilution of 1:10,000, whereas for (II) IgM detection, a polyclonal anti-porcine IgM antibody (#AAI48, Bio-Rad) was added at a dilution of 1:5,000. .. ELISA TMB Stabilized Chromogen (ThermoFisher) was added as substrate.



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    Antibody source, dilution, and antigen retrieval for immunohistochemistry to assess swine PCs producing IgG (a), IgM (b) and IgA (c) and the sandwich immunohistochemical protocol (d) for the detection of anti-PCV2 antibody-producing PCs.

    Journal: Veterinary Immunology and Immunopathology

    Article Title: Quantitative immunohistochemical assessment of IgA, IgM, IgG and antigen-specific immunoglobulin secreting plasma cells in pig small intestinal lamina propria

    doi: 10.1016/j.vetimm.2014.05.014

    Figure Lengend Snippet: Antibody source, dilution, and antigen retrieval for immunohistochemistry to assess swine PCs producing IgG (a), IgM (b) and IgA (c) and the sandwich immunohistochemical protocol (d) for the detection of anti-PCV2 antibody-producing PCs.

    Article Snippet: Primary antibody , Rabbit polyclonal anti-porcine IgG (Novus Biologicals Europe); 1:40,000 in PBS + 20% normal goat serum; overnight at 5 ± 3 °C , Rabbit polyclonal anti-porcine IgM (Novus Biologicals Europe); 1:40,000 in PBS + 20% normal goat serum; overnight at 5 ± 3 °C , Goat polyclonal anti-porcine IgA (Novus Biologicals Europe); 1:4,000 in PBS + 1% BSA; overnight at 5 ± 3 °C , Anti-PCV2 monoclonal antibody (F217, provided by G. Allan, Belfast, UK); 1:200 in PBS; 37 °C for 2 hours.

    Techniques: Immunohistochemistry, Immunohistochemical staining, Blocking Assay, Suspension